Binding Assay:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Generated:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Biomarker Discovery:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Derivative Assay:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Concentration Assay:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Negative Control:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Positive Control:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Standard Deviation:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Fluorescence:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
In Vitro:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Activity Assay:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Control:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Size-exclusion Chromatography:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Purification:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Comparison:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Two Tailed Test:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Western Blot:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Expressing:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Over Expression:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Phospho-proteomics:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Activation Assay:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Ligand Binding Assay:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Residue:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Inhibition:Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a first-in-class dual-function TREM2 modulator: Receptor activation and shedding inhibition.
Article Snippet: The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).The interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8 K instrument (Cytiva).. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5 % Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4343 ± 399 RU.. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: Structure-Based Virtual Screening Identifies TREM2-Targeted Small Molecules that Enhance Microglial Phagocytosis
Article Snippet: of EN020 to human TREM2 protein was assessed using surface plasmon resonance (SPR) on a Biacore 8K system (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084- H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, 0.5% Surfactant P20, pH 7.4) to a level of 4,343 ± 399 RU. .. Prior to immobilization, the chip surface was conditioned with three 1- minute injections of 1 M NaCl in 50 mM NaOH.
Article Title: As48, a First-in-Class Dual-Function TREM2 Modulator: Receptor Activation and Shedding Inhibition
Article Snippet: interaction between As48 and the human TREM2 protein was evaluated using surface plasmon resonance (SPR) on a Biacore 8K instrument (Cytiva). .. Biotinylated TREM2 (Cat. No. 11084-H49H-B, Sino Biological) was immobilized on an SA Sensor Chip (Cytiva) in PBS-P buffer (0.2 M phosphate buffer, 27 mM KCl, 1.37 M NaCl, and 0.5% Surfactant P20, pH 7.4; Cytiva) to a response level of RU 4,343 ± 399 RU. .. Before protein immobilization, the chip surface was conditioned with three 1-minute injections of 1 M NaCl in 50 mM NaOH.
|
Sino Biological is a verified supplier 
Sino Biological manufactures this product 
